General Cell Based Assays
Enzo Life Sciences FLUOR DE LYS-Green SIRT5 fluorometric drug discovery assay kit (100 tests)
The FLUOR DE LYS Green SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats. The Green SIRT5 Fluorescent Activity Assay is based on the unique FLUOR DE LYS-Succinyl GreenSubstrate/Developer combination. The FLUOR DE LYS-Succinyl GreenSubstrate, which comprises a lysine residue, Nε-succinylated on its side-chains, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS-Succinyl Green sensitizes it so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Green sirtuin 5 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
Molecular Devices QBT Fatty Acid Uptake Assay, Bulk, BODIPY-dodecanoic, 96/384-well
The QBT Fatty Acid Uptake Assay Kit is a homogeneous, fluorescence-based assay for measuring live-cell fatty acid uptake kinetics and screening fatty acid transporters in high-throughput formats. The assay uses a fluorescent fatty acid analog with a quench reagent to reduce extracellular background and enable kinetic readings.
- Homogeneous fluorescence assay suitable for high-throughput screening.
- Uses a BODIPY-dodecanoic fluorescent fatty acid analog and quench reagent.
- Bulk kit contains 10 vials, sufficient for approximately 50 plates.
- Compatible with 96-well and 384-well microplate formats.
- Measure kinetics with bottom-read fluorescence, excitation ~470-500 nm, emission ~500-560 nm.
- Store kit at -20°C; formulated loading buffer stable up to 8 hours at room temperature.
Eurofins Discoverx PathHunter eXpress PTGFR Total Internalization Assay, 2pl Kit. (93-0872E3CP0M)
The PathHunter PTGFR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGFR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGFR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGFR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCXCR7 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0689E3MCP5L)
The PathHunter mCXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR7 and EA-tagged β-Arrestin-2. Activation of mCXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human IFN beta (IFNAR1/IFNAR2) Dimerization Cell-Based Assay Kit (HEK 293)
The PathHunter Human IFN beta (IFNAR1/IFNAR2) Stable Cell Line Dimerization Assay (HEK 293) measures ligand-induced dimerization of IFNAR1/IFNAR2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical 2-bromo 17β-Estradiol, Purity: ≥98%, CAS: 15833-07-5, 5 mg
A synthetic steroid and inhibitor of estrogen 2-hydroxylase; inhibits estrogen 2-hydroxylase in rat liver microsomes at 50 and 100 µM; prevents estrogen-induced inhibition of apomorphine-induced climbing behavior in mice at 5 mg/kg
Abcam Soluble Epoxide Hydrolase Assay Kit
Soluble Epoxide Hydrolase Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter Human PROKR2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PROKR2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PROKR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
10X Genomics Inc GEM-X Flex Gene Expression Mouse n-plex, 64 samples
GEM-X Flex Gene Expression Mouse n-plex, 64 samples
Eurofins Discoverx PathHunter eXpress CHRM3 Total Internalization Assay, 10pl Kit. (93-0775E3CP5L)
The PathHunter CHRM3 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM3 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CHRM3 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CHRM3 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Pyruvate Kinase PK from ra
ATPpyruvate 2-O-phosphotransferasePyruvate kinase has a molar mass of 237 000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57 200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2 and phosphoenolpyruvate.
Eurofins Discoverx PathHunter eXpress P2RY11 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0281E2CP2M)
The PathHunter P2RY11 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ATP) based activation of P2RY11 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY11 and EA-tagged β-Arrestin-2. Activation of P2RY11 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BioVendor, LLC FASTIN ELASTIN 110 ASSAYS STNDSZE
TypeColorimetric assayApplicationsCell culture and/or animal studies, Tissue extract, CellSample RequirementsA sample volume of between 50 and 500 μl is required, containing not less than 5 μg and not more than 70 μg elastin.ShippingAt ambient temperature. Upon receipt, store the product at the temperature recommended below.Storage/ExpirationStore the kit at RT or 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box). Calibration Range5 – 70 μgLimit of Detection5 μg
Eurofins Discoverx PathHunter Mouse EDNRA β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse EDNRA β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mouse EDNRA (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.